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rajendar nallapu senior biotechnologist

uae
biotechnology in production division

 

RAJENDAR.N

M: +971554965305, E-Mail: [email protected]

Gulf pharmaceuticals Ras Al khaimah UAE PO BOX NO 997
JOB PROFILE

9 years of strong industrial experience in downstream process. (Production & process development of insulin and glargine molecules.
·         Strong  experience in chromatography system and TFF system of various make and scale.

·         Prioritizing in experiment design and process optimization.

 

 

GULF PHARMACEUTICALS (JULPHAR), UAE

 

Designation: Senior production biotechnologist

Department: Production (Downstream process)

MARCH 2012 – Till date

CAREER PROGRESSION

 

 

 

 

 

Responsibilities:

 

Ø       Process and development of recombinant protein molecule (Insulin).

Ø       Validation of process and cleaning.

Ø       Optimization of TFF process.

Ø       Optimization of centrifuge process as per process requirements.

Ø       Operation of lyophilizer.

Ø       Involved in optimization of lyophilization process as per working molecule.

Ø       SOP preparation.

Ø       Online completion, review and submission of MFM.

Ø       Involved in pilot scale experiments as per process development.

Ø       Autoclave and DHS validation as per process requirements.

Ø       Analyzing the problem and troubleshooting.

 

 

VIVO BIOTECH HYDERBAD

 

Designation: Research Assistant

Department: Downstream process and cell biology

FEB 2008 – JAN 2012

 

 

 

 

 
Responsibilities:

 

Ø  Protein Purification:

 

Ø Cell Lysis methods: Enzymatic, Sonication.

Ø Inclusion Bodies isolation, purification and solubilisation.

Ø Concentration and purification of Proteins by TFF and Dialysis

Ø Protein refolding methods.

Ø Chromatography methods.

 

 

 

 

Ø  Analytical method development:

 

Ø  Electrophoresis: SDS-PAGE, Isoelectric focusing.

Ø  Immunoblotting: Western blot.

Ø  Quantitative protein estimation method

Ø  UV/VIS Spectrophotometry.

 

Ø  Microbiology:

 

Ø  Shake flask culture

 

Ø  Molecular Biology and cell biology:

 

Ø  Microscopy

Ø  Preparation of different media for mammalian cell lines

Ø  Animal Cell Culture techniques

Ø  Primary and secondary cell culturing

Ø  Isolation of DNA, RNA, Plasmid

Ø  Viability assays like MTT Assay, XTT assay Cell counting using Haemocytometer

Ø  Enzymatic assay

Ø  AGE

Ø  Western Blotting

Ø  SDS PAGE

Ø  ELISA

Ø  Handling of different mammalian cell lines

Ø  Cryopreservation and revival of cryopreserved mammalian cells lines.

Ø  In vitro Biological activity determination of recombinant proteins by mammalian cell line

Ø  Buffer preparation

Ø  Preparation of cell culture reagents, antibiotics

 

UNIVERSITY OF HYDERABAD

 

Designation: Junior Research Fellow

Department: PLANT MOLECULAR BIOLOGY

FEB 2005 – APRIL 2007

 

 

 

 

 

Ø  Responsibilities:

Ø   Isolation of DNA, RNA, Plasmid.

Ø  Gene transformation methods in plant and Bacteria.

Ø  PCR, RAPD, RFLP.

 

 

 

 

 

 

 

 

 

 

EQUIPMENT AND INSTRUMENT HANDLING

Ø  AKTA Pilot, Purifier, Bioprocessor.           :              GE

Ø  Chromatography system and column     :               Pall and GE.

Ø  TFF system                                                         :               Pall and GE.

Ø  Centrifuge                                                          :               GEA

Ø  Lyophilizer                                                          :               Martin Christ.

Ø  Integrity Test machine                                   :               Pall.

Ø  Operation of HMI and DCS

 

ACADEMIC QUALIFICATION

 

Ø  M.Sc., (Microbiology) in 2000 – 2002 with – 71% from Bangalore University.

Ø  B.Sc., (Microbiology) in 1997 – 2000 with – 69% from Kakathiya University.

Ø   Board of Intermediate (BiPC) in 1996 – 1997.

 

 

PERSONAL DETAILS

 

Name                                : Rajendar.N

Father Name                     : Keshavulu

Date of birth                      : 11.01.1976

Marital status                     : Married

Nationality                         : Indian

Permanent Address          : Post nagaram, mandal Hasanparthy, Dt, Warangal, Telangana, India

 

 

REFERENCE:

Ø    Mr. KANNAN,

Purification manager,

Gulf pharmaceuticals (Juphar XI)

RAK, UAE.

Mail Id: [email protected]

Ph.no:+971553236213

 

 

 

 

 

 

 

 

 

 

 

 

DECLARATION

The information furnished above is true to best of my knowledge and belief.

 

 

N.RAJENDAR

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

s

 

Education

aug 2000 to aug 2002 masters in biotechnology at bangalore university

anywhere biotechnology protein purification division

Experience

2012 march to till date senior biotechnologist at gulf pharmaceuticals

before having five years experiance at india